SKU: 21-1026
{"url":"https://www.epicypher.com/products/epigenetics-kits-and-reagents/cutana-nuclei-extraction-buffer","add_this":[{"service":"facebook","annotation":""},{"service":"email","annotation":""},{"service":"print","annotation":""},{"service":"twitter","annotation":""},{"service":"linkedin","annotation":""}],"gtin":null,"options":[],"id":1121,"bulk_discount_rates":[],"can_purchase":true,"meta_description":"CUTANA™ Nuclei Extraction Buffer includes all needed reagents to extract nuclei from cells in preparation for genomic mapping assays","category":["Epigenetics Kits and Reagents","Epigenetics Kits and Reagents/CUTANA™ ChIC / CUT&RUN Assays","Epigenetics Kits and Reagents/CUTANA™ CUT&Tag Assays"],"AddThisServiceButtonMeta":"","main_image":{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1121/1183/Screen_Shot_2020-02-12_at_11__54081.1697176206.jpg?c=2","alt":"CUTANA™ Nuclei Extraction Buffer"},"add_to_wishlist_url":"/wishlist.php?action=add&product_id=1121","shipping":{"calculated":true},"num_reviews":0,"weight":"0.01 LBS","custom_fields":[{"id":"1283","name":"Pack Size","value":"100 Reactions"}],"sku":"21-1026","description":"<div class=\"product-general-info\">\n <ul style=\"display: none\" class=\"product-general-info__list-left\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"display: none\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\"></li>\n <li class=\"product-general-info__list-item\"></li>\n </ul>\n <ul style=\"padding: 0\" class=\"product-general-info__list-right\">\n <li class=\"product-general-info__list-item\">\n <a href=\"#bioz\">\n <div\n id=\"w-s-3835-21-1026\"\n style=\"\n width: max-content;\n height: 58px;\n position: relative;\n overflow-y: hidden;\n \"></div>\n <div id=\"bioz-w-pb-21-1026-div\">\n <a\n id=\"bioz-w-pb-21-1026\"\n style=\"font-size: 12px; color: transparent\"\n href=\"https://www.bioz.com/\"\n target=\"_blank\">\n <img\n src=\"https://cdn.bioz.com/assets/favicon.png\"\n style=\"\n width: 11px;\n height: 11px;\n vertical-align: baseline;\n padding-bottom: 0px;\n margin-left: 0px;\n margin-bottom: 0px;\n float: none;\n display: none;\n \" />\n </a></div\n ></a>\n </li>\n </ul>\n</div>\n<div class=\"service_accordion product-droppdown\">\n <div class=\"container\">\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel current\">\n <h3 class=\"sub-title1\">Description</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <p>\n CUTANA<sup>™</sup> Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in\n CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are\n examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer:\n </p>\n\n <table class=\"epicypher-table\">\n <tr>\n <td>mouse NIH3T3 fibroblast cells</td>\n <td> human A549 non-small cell lung cancer (NSCLC) cells</td>\n </tr>\n <tr>\n <td>human K562 leukemia cells </td>\n <td>human NCI-H1299 non-small cell lung cancer (NSCLS cells)</td>\n </tr>\n <tr>\n <td>human bone marrow derived macrophages</td>\n <td>human TIG-1 fetal lung cells </td>\n </tr>\n <tr>\n <td>human monocyte derived macrophages </td>\n <td>human LoVo colorectal cancer cells</td>\n </tr>\n <tr>\n <td>human MV-4-11 macrophage cells</td>\n <td>human LNCaP prostate carcinoma cells</td>\n </tr>\n <tr>\n <td>human SUM149 triple negative breast cancer (TNBC) cells</td>\n <td>human renal primary cells</td>\n </tr>\n <tr>\n <td>human GM24385 B-lymphocyte (aka HG002) cells</td>\n <td>human peripheral blood mononuclear cells (PBMCs)</td>\n </tr>\n <tr>\n <td>human MCF7 breast cancer cells</td>\n <td>human intestinal tissue</td>\n </tr>\n <tr>\n <td>human MDA-MB-231 breast cancer cells </td>\n <td>human HEPM embryonic cells</td>\n </tr>\n <tr>\n <td>human SK-MEL-2 melanoma cells</td>\n <td></td>\n </tr>\n </table>\n\n <p>\n The reagent is prepared by supplementing Pre-Nuclei Extraction Buffer with protease inhibitor and spermidine fresh on the\n day of use. Utilize this buffer in EpiCypher’s <a href=\"https://support.epicypher.com/docs/nuclei-extraction-protocol-for-cutana-assays\">CUTANA<sup>™</sup> Nuclei Extraction Protocol for CUT&RUN and CUT&Tag</a>\n to obtain the highest quality nuclei for your genomic mapping assay.\n </p>\n\n </div>\n </div>\n </div>\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Validation Data</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description-specific\">\n <section class=\"image-picker\">\n <div class=\"image-picker__left\">\n <div class=\"image-picker__main-content_active image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/21-1026-23249001-01-Figure-1.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"21-1026-23249001-01-Figure-1.jpg\"\n src=\"/content/images/products/nucleosomes/21-1026-23249001-01-Figure-1.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 1: Nuclei Extraction</strong\n ><br />\n Nuclei were extracted from 3 different cell\n types: K562, NIH3T3, and LNCaP using the CUTANA<sup>™</sup> Nuclei Extraction\n Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before\n extraction. Starting cells are viable (bright white and round). Bottom 3\n panels show nuclei extracted using the CUTANA<sup>™</sup> Nuclei Extraction Buffer\n as indicated by positive Trypan Blue staining.\n </span>\n </p>\n </div>\n <!-- <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/14_1001_distribution_analysis.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\"\n ><img\n loading=\"lazy\"\n alt=\"14_1001_distribution_analysis\"\n src=\"/content/images/products/nucleosomes/14_1001_distribution_analysis.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n ></a\n >\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\"\n ><strong\n >Figure 2: CUT&RUN DNA Fragment Size Distribution\n Analysis</strong\n ><br />\n CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit\n (EpiCypher\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n >14-1048</a\n >) starting with 500k K562 cells. Five nanograms of CUT&RUN\n output DNA from reactions utilizing IgG (EpiCypher\n <a\n href=\"/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody\"\n >13-0042</a\n >), H3K4me3 (EpiCypher\n <a\n href=\"/products/antibodies/snap-chip-certified-antibodies/histone-h3k4me3-antibody-snap-chip-certified-cutana-cut-run-compatible\"\n >13-0041</a\n >), H3K27me3 (ABclonal A16199), and CTCF (EpiCypher\n <a\n href=\"/products/antibodies/cutana-cut-run-compatible-antibodies/ctcf-cutana-cut-and-run-antibody\"\n >13-2014</a\n >) antibodies were prepared for paired-end Illumina<sup\n >®</sup\n >\n sequencing with the CUTANA CUT&RUN Library Prep Kit. Library\n DNA was analyzed by Agilent TapeStation<sup>®</sup>, which\n confirmed that mononucleosomes were predominantly enriched\n in CUT&RUN (~300 bp peaks represent 150 bp nucleosomes +\n sequencing adapters). Peaks at ~380 bp correspond to the\n SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher\n <a href=\"/products/nucleosomes/snap-cutana-k-metstat-panel\"\n >19-1002</a\n >).\n </span>\n </p>\n </div>\n <div class=\"image-picker__main-content\">\n <div class=\"image-picker__header-content\">\n <button class=\"image-picker__left-arrow\">\n <svg\n class=\"image-picker__svg-left\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M16.67 0l2.83 2.829-9.339 9.175 9.339 9.167-2.83 2.829-12.17-11.996z\" />\n </svg>\n </button>\n <a\n href=\"/content/images/products/nucleosomes/14_1001_target_compatibility.jpg\"\n target=\"_new\"\n class=\"image-picker__main-image-link\">\n <img\n loading=\"lazy\"\n alt=\"14_1001_target_compatibility\"\n src=\"/content/images/products/nucleosomes/14_1001_target_compatibility.jpg\"\n class=\"image-picker__main-image\" />\n <span class=\"image-picker__main-image-caption\"\n >(Click to enlarge)</span\n >\n </a>\n <button class=\"image-picker__right-arrow\">\n <svg\n class=\"image-picker__svg-right\"\n width=\"24\"\n height=\"24\"\n viewBox=\"0 0 24 24\">\n <path\n d=\"M7.33 24l-2.83-2.829 9.339-9.175-9.339-9.167 2.83-2.829 12.17 11.996z\" />\n </svg>\n </button>\n </div>\n <p>\n <span class=\"image-picker__span-content\">\n <strong>Figure 3: Representative gene browser tracks</strong\n ><br />\n\n CUT&RUN sequencing libraries described in Figure 2 were\n sequenced on an Illumina<sup>®</sup> NextSeq 2000 with a P3\n cartridge (paired-end 2x50 cycle). Data were aligned to the\n hg19 genome using Bowtie2. Data were filtered to remove\n duplicates, multi-aligned reads, and blacklist regions. A\n representative 640 kb window at the SEPTIN5 gene is shown\n for three replicates (\"Rep\") of IgG and H3K4me3 antibodies,\n as well as individual tracks for H3K27me3 and the\n transcription factor CTCF, demonstrating the robustness and\n reproducibility of the workflow with a variety of targets.\n Sequencing libraries prepared with the CUTANA CUT&RUN\n Library Prep kit produced the expected genomic distribution\n for each target. Sample sequencing depth was as follows\n (millions of reads): IgG Rep 1 (20.1), IgG Rep 2 (16.6), IgG\n Rep 3 (16.1), H3K4me3 Rep 1 (7.3), H3K4me3 Rep 2 (17.2),\n H3K4me3 Rep 3 (13.8), H3K27me3 (13.4), CTCF (16.2). Images\n were generated using the Integrative Genomics Viewer (IGV,\n Broad Institute).\n </span>\n </p>\n </div> -->\n </div>\n <aside class=\"image-picker__right\">\n <div class=\"image-picker__gallery\">\n <img\n loading=\"lazy\"\n alt=\"21-1026-23249001-01-Figure-1.jpg\"\n src=\"/content/images/products/nucleosomes/21-1026-23249001-01-Figure-1.jpg\"\n width=\"200\"\n class=\"image-picker__side-image image-picker__side-image_active\"\n role=\"button\" />\n <!-- <img\n loading=\"lazy\"\n alt=\"14_1001_distribution_analysis\"\n src=\"/content/images/products/nucleosomes/14_1001_distribution_analysis.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" />\n <img\n loading=\"lazy\"\n alt=\"14_1001_target_compatibility\"\n src=\"/content/images/products/nucleosomes/14_1001_target_compatibility.jpg\"\n class=\"image-picker__side-image\"\n role=\"button\" /> -->\n </div>\n </aside>\n </section>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Contents</h3>\n <div class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> Pre-Nuclei Extraction Buffer </td>\n <td>\n <!-- <a href=\"/products/epigenetics-kits-and-reagents/cutana-chic-cut-run-assays/cutana-cut-run-8-strip-0-2-ml-tubes\"> -->\n 21-1026a\n <!-- </a> -->\n </td>\n </tr>\n\n <tr>\n <td>1 M Spermidine</td>\n <td>21-1026b</td>\n </tr>\n </table>\n </div>\n </div>\n </div>\n \n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Required Materials Not Supplied</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <p>\n Protease inhibitor is not included. EpiCypher suggests using CUTANA<sup>™</sup> Protease Inhibitor Tablets (<a href=\"/products/epigenetics-kits-and-reagents/cutana-protease-inhibitor-tablets\" target=\"_blank\">21-1027</a>).\n </p>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Recommended Accessory Products</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <table class=\"epicypher-table\">\n <tr>\n <th>Item</th>\n <th>Cat. No.</th>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> ChIC/CUT&RUN Kit </td>\n <td>\n <a\n href=\"/products/epigenetics-reagents-and-assays/cutana-chic-cut-and-run-kit\"\n style=\"text-decoration: none;\" >\n 14-1048\n </a>\n </td>\n </tr>\n\n <tr>\n <td>CUTANA<sup>™</sup> pAG-MNase for ChIC/CUT&RUN </td>\n <td>\n <a style=\"text-decoration: none;\" href=\"/products/epigenetics-reagents-and-assays/cutana-pag-mnase-for-chic-cut-and-run-workflows\">\n 15-1016/15-1116\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> pAG-Tn5 for CUT&Tag</td>\n <td>\n <a style=\"text-decoration: none;\" href=\"/products/epigenetics-kits-and-reagents/cutana-pag-tn5-for-cut-and-tag\">\n 15-1017/15-1117\n </a>\n </td>\n </tr>\n\n <tr>\n <td id=\"specific-row\">CUTANA<sup>™</sup> Protease Inhibitor Tablets</td>\n <td>\n <a style=\"text-decoration: none;\" href=\"/products/epigenetics-kits-and-reagents/cutana-protease-inhibitor-tablets\">\n 21-1027\n </a>\n </td>\n </tr>\n </table>\n\n <p>\n <strong> NOTE: </strong>\n CUTANA<sup>™</sup> CUT&Tag Kit (<a href=\"/products/epigenetics-kits-and-reagents/cutana-cut-and-tag-kit\">14-1102/14-1103</a>) already contains Nuclei Extraction Buffer\n </p>\n </div>\n </div>\n </div>\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <h3 class=\"sub-title1\">Technical Information</h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-tech-info\">\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Storage</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n Store the Pre-Nuclei Extraction Buffer at 4ºC and the 1 M Spermidine at -20ºC. Components are\n stable for 6 months upon date of receipt.\n </br><p></p>\n </div>\n </div>\n <div class=\"product-tech-info__line-item\">\n <div class=\"product-tech-info__line-item-left\">\n <b>Instructions for Use</b>\n </div>\n <div class=\"product-tech-info__line-item-right\">\n <p> \n Prepare Nuclei Extraction Buffer FRESH on day of use as outlined below. This master mix includes\n 20% extra volume to account for pipetting error; no additional buffer volume is needed. \n </p>\n <p> Per CUT&RUN or CUT&Tag reaction, combine:</p>\n \n <p style=\"margin-bottom: 0;\"> 235 µL Pre-Nuclei Extraction Buffer (21-1026a) </p>\n <p style=\"margin-bottom: 0;\">0.13 µL 1M Spermidine (21-1026b)* </p>\n <p>\n 9.8 µL 25X Protease Inhibitor (Prepare using CUTANA<sup>™</sup> Protease Inhibitor Tablets, <a href=\"/products/epigenetics-kits-and-reagents/cutana-protease-inhibitor-tablets\" target=\"_blank\">21-1027</a>)**\n </p>\n\n <p style=\"margin-bottom: 0;\"><i>*If preparing buffer for fewer than 8x reactions, \n dilute the 1M Spermidine stock 1:10 in molecular biology-grade water and add 1.3 µL per reaction.</i> \n </p>\n <p> <i>**Leftover 25X Protease Inhibitor can be stored for 12 weeks at -20ºC </i></p>\n\n <p> Follow instructions detailed in EpiCypher’s <a href=\"/resources/protocols/\"> CUTANA<sup>™</sup> Nuclei Extraction Protocol for CUT&RUN and CUT&Tag</a> to isolate nuclei from suspension and adherent cells. The\n protocol includes key quality control checks to ensure nuclei are high quality before starting\n CUT&RUN or CUT&Tag.\n </p>\n </div>\n </div>\n </div>\n </div>\n </div>\n </div>\n\n\n <!-- <h3\n style=\"color: #4698cb; font-size: 24px; border-bottom: none\"\n class=\"custom-title-description\">\n Documents & Resources\n </h3> -->\n\n <div id=\"prodAccordion\">\n <div id=\"ProductDescription\" class=\"Block Panel\">\n <!-- <h3 style=\"color: #4698cb; margin-bottom: 0\">Current Lot</h3> -->\n <h3 class=\"sub-title1\">Documents & Resources </h3>\n <div\n class=\"ProductDescriptionContainer product-droppdown__section-description\">\n <div class=\"product-documents\">\n <a\n href=\"/content/documents/tds/21-1026.pdf\"\n target=\"_new\"\n class=\"product-documents__link\">\n <svg\n version=\"1.1\"\n id=\"Layer_1\"\n xmlns=\"http://www.w3.org/2000/svg\"\n xmlns:xlink=\"http://www.w3.org/1999/xlink\"\n x=\"0px\"\n y=\"0px\"\n viewBox=\"0 0 228 240\"\n style=\"enable-background: new 0 0 228 240\"\n xml:space=\"preserve\"\n class=\"product-documents__icon\"\n alt=\"16-0002 Datasheet\">\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M191.92,68.77l-47.69-47.69c-1.33-1.33-3.12-2.08-5.01-2.08H45.09C41.17,19,38,22.17,38,26.09v184.36\n c0,3.92,3.17,7.09,7.09,7.09h141.82c3.92,0,7.09-3.17,7.09-7.09V73.8C194,71.92,193.25,70.1,191.92,68.77z M177.65,77.06h-41.7\n v-41.7L177.65,77.06z M178.05,201.59H53.95V34.95h66.92v47.86c0,5.14,4.17,9.31,9.31,9.31h47.86V201.59z\" />\n </g>\n <rect\n x=\"20\"\n y=\"112\"\n class=\"product-documents__svg-background\"\n width=\"146\"\n height=\"76\" />\n <g>\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M23.83,125.68h22.36c5.29,0,9.41,1.33,12.35,4c2.94,2.67,4.42,6.39,4.42,11.18c0,4.78-1.47,8.51-4.42,11.18\n c-2.94,2.67-7.06,4-12.35,4H34.59v18.29H23.83V125.68z M44.81,147.9c5.38,0,8.07-2.32,8.07-6.97c0-2.39-0.67-4.16-2-5.31\n c-1.33-1.15-3.36-1.73-6.07-1.73H34.59v14.01H44.81z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M69.92,125.68h18.91c5.29,0,9.84,0.97,13.66,2.9c3.82,1.93,6.74,4.72,8.76,8.35\n c2.02,3.63,3.04,7.98,3.04,13.04c0,5.06-1,9.42-3,13.08c-2,3.66-4.91,6.45-8.73,8.38c-3.82,1.93-8.4,2.9-13.73,2.9H69.92V125.68z\n M88.07,165.63c10.35,0,15.52-5.22,15.52-15.66c0-10.4-5.17-15.59-15.52-15.59h-7.38v31.26H88.07z\" />\n <path\n class=\"product-documents__svg-pdf\"\n d=\"M122.57,125.68h32.84v8.49h-22.22v11.18h20.84v8.49h-20.84v20.49h-10.63V125.68z\" />\n </g>\n </svg>\n <span class=\"product-documents__info\"\n >Technical Datasheet</span>\n </a>\n</div>\n<div class=\"product-documents\">\n<a\n href=\"https://support.epicypher.com/article/36-cutana-nuclei-extraction-protocol?_gl=1*1t9jw12*_gcl_aw*R0NMLjE2OTcwNTc4NjIuQ2owS0NRand3dmlsQmhDRkFSSXNBRHZZaTdLdzhXM2pXXzBuWWFjNVUxdXdYMmtRYWhBQV85VjdmZWRjUzdDRnJrMi1ndzk0aTdEaXdXVWFBbmh0RUFMd193Y0I.*_gcl_au*MzU2MzU3MTQyLjE2OTQ2MjYwMDg\"\n target=\"_new\"\n class=\"product-documents__link\">\n<svg fill=\"#f4bf52\" class=\"product-documents__icon\" viewBox=\"0 0 24 24\" xmlns=\"http://www.w3.org/2000/svg\"><g id=\"SVGRepo_bgCarrier\" stroke-width=\"0\"></g><g id=\"SVGRepo_tracerCarrier\" stroke-linecap=\"round\" stroke-linejoin=\"round\"></g><g id=\"SVGRepo_iconCarrier\"> <g data-name=\"Layer 2\"> <g data-name=\"link-2\"> <rect width=\"24\" height=\"24\" opacity=\"0\"></rect> <path fill=\"#f4bf52\" d=\"M13.29 9.29l-4 4a1 1 0 0 0 0 1.42 1 1 0 0 0 1.42 0l4-4a1 1 0 0 0-1.42-1.42z\"></path> <path d=\"M12.28 17.4L11 18.67a4.2 4.2 0 0 1-5.58.4 4 4 0 0 1-.27-5.93l1.42-1.43a1 1 0 0 0 0-1.42 1 1 0 0 0-1.42 0l-1.27 1.28a6.15 6.15 0 0 0-.67 8.07 6.06 6.06 0 0 0 9.07.6l1.42-1.42a1 1 0 0 0-1.42-1.42z\" fill=\"#f4bf52\"></path> <path fill=\"#f4bf52\" d=\"M19.66 3.22a6.18 6.18 0 0 0-8.13.68L10.45 5a1.09 1.09 0 0 0-.17 1.61 1 1 0 0 0 1.42 0L13 5.3a4.17 4.17 0 0 1 5.57-.4 4 4 0 0 1 .27 5.95l-1.42 1.43a1 1 0 0 0 0 1.42 1 1 0 0 0 1.42 0l1.42-1.42a6.06 6.06 0 0 0-.6-9.06z\"></path> </g> </g> </g></svg>\n <span class=\"product-documents__info\"\n >CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag</span>\n </a>\n </div>\n </div>\n </div>\n </div>\n \n </div>\n</div>\n\n<script>\n $(document).ready(function () {\n var widget_micro_obj = new v_widget_obj('s', [1]);\n widget_micro_obj.request_catalog_number_widget_data_internal(\n '21-1026',\n '21-1026'\n );\n });\n</script>\n\n<style>\n .form-field-title .required-text {\n display: none !important;\n }\n\n .form-label {\n padding-left: 2rem;\n }\n\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n /* //////////// */\n\n /* BIOZ */\n td table {\n margin: 0;\n padding: 6px !important;\n }\n\n span.bioz-w-parent-hover {\n font-size: 15px;\n }\n\n td .bioz-w-tooltipx {\n padding-top: 0 !important;\n }\n /* .bioz-w-parent-hover:hover {\n text-decoration: underline !important;\n } */\n\n /* /////////// */\n\n .form-field-title .required-text {\n display: none !important;\n }\n .form-label {\n padding-left: 2rem;\n }\n .form-label-text {\n margin: 0;\n margin-left: 0 !important;\n }\n\n .bioz-w-header td {\n padding: 0;\n }\n\n .epicypher-table table {\n margin-right: auto;\n margin-left: auto;\n width: 100%;\n border-top: 1px solid lightgray;\n }\n\n .epicypher-table th {\n color: #3b3a48;\n }\n .epicypher-table td {\n color: #3b3a48;\n font-size: 0.9rem;\n }\n .epicypher-table td a {\n text-decoration: underline;\n }\n .epicypher-table td,\n th {\n border: 0.5px solid lightgray;\n text-align: left;\n padding: 8px;\n /* white-space: nowrap; */\n }\n .epicypher-table tr:nth-child(even) {\n background-color: #f9f9f9;\n }\n .epicypher-table td:first-child {\n border-left: 1px solid lightgray;\n }\n\n /* Tablet */\n\n @media only screen and (max-width: 1024px) {\n .epicypher-table th {\n display: none !important;\n }\n\n /* BIOZ */\n td.bioz-w-tooltipx::before {\n display: none;\n }\n\n /* Hide table headers (but not display: none;, for accessibility) */\n .epicypher-table thead tr {\n position: absolute;\n top: -9999px;\n left: -9999px;\n }\n\n .epicypher-table tr {\n border: 1px solid #ccc;\n }\n\n .epicypher-table td {\n /* Behave like a \"row\" */\n /* border: none; */\n border-bottom: 1px solid #eee;\n position: relative;\n padding-left: 30%;\n white-space: inherit;\n }\n\n .epicypher-table td:before {\n /* Now like a table header */\n position: absolute;\n /* Top/left values mimic padding */\n top: 6px;\n left: 6px;\n width: 45%;\n padding-right: 10px;\n white-space: nowrap;\n font-weight: 700;\n }\n\n /*\n Label the data\n */\n .epicypher-table td:nth-of-type(1):before {\n content: 'Item';\n }\n td:nth-of-type(2):before {\n content: 'Cat. No.';\n }\n }\n\n @media screen and (min-width: 768px) {\n .products-featured .container,\n .products-featured .product-tabs,\n .products-related .container,\n .products-related .product-tabs {\n padding-left: 30px !important;\n padding-right: 50px;\n max-width: 1170px !important;\n }\n .bioz-w-header,\n .bioz-w-parent-hover td table tbody tr {\n display: inherit;\n }\n }\n\n @media screen and (max-width: 767px) {\n .custom-title-description {\n font-size: 19px !important;\n }\n .bioz-w-header,\n .bioz-w-parent-hover td table tbody tr {\n display: flex;\n flex-direction: row;\n }\n\n .section-title {\n font-size: 19px !important;\n padding-left: 1rem;\n }\n }\n</style>\n","tags":[],"warranty":"","price":{"without_tax":{"formatted":"$295.00","value":295,"currency":"USD"},"tax_label":"Sales Tax"},"detail_messages":"","availability":"","page_title":"CUTANA™ Nuclei Extraction Buffer","cart_url":"https://www.epicypher.com/cart.php","max_purchase_quantity":0,"mpn":null,"upc":null,"shipping_messages":[],"rating":0,"meta_keywords":"CUTANA, CUT&RUN, CUT and RUN, CUT&Tag, CUT and Tag, Nuclei Extraction, Nuclei Buffer, genomic mapping","show_quantity_input":1,"title":"CUTANA™ Nuclei Extraction Buffer","gift_wrapping_available":false,"min_purchase_quantity":0,"customizations":[],"images":[{"data":"https://cdn11.bigcommerce.com/s-y9o92/images/stencil/{:size}/products/1121/1183/Screen_Shot_2020-02-12_at_11__54081.1697176206.jpg?c=2","alt":"CUTANA™ Nuclei Extraction Buffer"}]}
Pack Size: 100 Reactions
